Free radical scavenging potential of Lagenaria siceraria (Molina) Standl fruits extract

Asian Pac J Trop Med. 2013 Jan;6(1):20-6. doi: 10.1016/S1995-7645(12)60195-3.

Abstract

Objective: To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria (L. siceraria) (Molina) fruit.

Methods: The free radical scavenging activity of the L. siceraria (Molina) fruit extract was assayed by using α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,20-azinobis 3-ethyl benzothiazoline-6-sulfonate (ABTS), FRAP, reducing power, chelating ability and β-carotene bleaching assay.

Results: The IC(50) values of DPPH and ABTS radical-scavenging activity was found to be 1.95 mg/mL and 19 mg/mL, respectively. In ferrous chelation assay, the percentage of inhibition was found to be 89.21%. The reducing power of ethanolic extract of L. siceraria (Molina) fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL. The β-carotene linoleate bleaching assay was 46.7% at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL.

Conclusions: The results indicate that L. siceraria (Molina) fruit could be an important sources of natural radical scavengers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Benzothiazoles
  • Biphenyl Compounds
  • Cucurbitaceae / chemistry*
  • Fruit / chemistry*
  • Iron Chelating Agents / chemistry
  • Iron Chelating Agents / pharmacology
  • Oxidation-Reduction / drug effects
  • Picrates
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Sulfonic Acids
  • Thiazoles
  • beta Carotene / chemistry

Substances

  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Iron Chelating Agents
  • Picrates
  • Plant Extracts
  • Sulfonic Acids
  • Thiazoles
  • beta Carotene
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • 1,1-diphenyl-2-picrylhydrazyl