A new strategy for the selective determination of glutathione in the presence of nicotinamide adenine dinucleotide (NADH) using a novel modified carbon nanotube paste electrode

A A Ensafi; Hassan Karimi-Maleh; S Mallakpour

doi:10.1016/j.colsurfb.2012.12.011

A new strategy for the selective determination of glutathione in the presence of nicotinamide adenine dinucleotide (NADH) using a novel modified carbon nanotube paste electrode

Colloids Surf B Biointerfaces. 2013 Apr 1:104:186-93. doi: 10.1016/j.colsurfb.2012.12.011. Epub 2012 Dec 21.

Authors

A A Ensafi¹, Hassan Karimi-Maleh, S Mallakpour

Affiliation

¹ Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Ensafi@cc.iut.ac

PMID: 23314609
DOI: 10.1016/j.colsurfb.2012.12.011

Abstract

A novel electrochemical sensor for the simultaneous determination of glutathione (GSH) and nicotinamide adenine dinucleotide (NADH) is described. The sensor is based on a carbon paste electrode (CPE) modified with benzamide derivative and multiwall carbon nanotubes. This mixture makes a modified electrode that is sensitive for the electrochemical detection of these compounds. Under optimum conditions and at pH 7.0, oxidation of GSH occurs at a potential of about 330 mV less positive than that at an unmodified CPE. The voltammetric peak currents are linearly dependent on GSH and NADH concentrations in the ranges 0.09-300 μmol L(-1) GSH and 5.0-600 μmol L(-1) NADH. The detection limits found for GSH and NADH were 0.05 μmol L(-1) and 1.0 μmol L(-1), respectively. The electrochemical sensor was also used for the determination of GSH in urine, pharmaceutical and hemolysed erythrocyte samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrochemical Techniques / instrumentation
Electrochemical Techniques / methods*
Electrodes
Glutathione / analysis*
NAD / chemistry*
Nanotubes, Carbon / chemistry*
Surface Properties

Substances

Nanotubes, Carbon
NAD
Glutathione