The aim of this study was to analyze the validity of 4 regression equations to estimate the peak oxygen consumption (V[Combining Dot Above]O2peak) from the 20-m shuttle run test in adolescents aged 11-13 years. One hundred and fifteen adolescents, 61 boys (mean ± SD: age = 12.3 ± 0.9 years) and 54 girls (age = 12.1 ± 0.7 years) performed the 20-m shuttle run test and an incremental progressive maximal test for direct V[Combining Dot Above]O2peak analysis. Four linear regression equations were used to estimate the V[Combining Dot Above]O2peak: Barnett et al. (equation 1), Léger et al. (equation 2), Mahar et al. (equation 3), and Matsuzaka et al. (equation 4). For boys, only the V[Combining Dot Above]O2peak estimated by EQ3 did not differ from the value directly measured (p > 0.05). The EQ1, EQ2, and EQ4 underestimated the V[Combining Dot Above]O2peak, whereas the EQ3 overestimated, particularly in girls (p < 0.05). Large limits of agreement were found between the reference method and the 4 equations, with higher estimated values by EQ2 for boys (8.36 ± 15.24 mL·kg·min) and girls (2.45 ± 12.63 mL·kg·min). The highest correlation values were observed by EQ4 for boys (r = 0.80), EQ1 for girls (r = 0.72), and EQ3 for total sample (r = 0.80). The equations analyzed were not precise for individual V[Combining Dot Above]O2peak prediction; however, the EQ3 revealed better agreement, particularly for boys. Considering the data obtained in the boys and total sample, our results suggest that the EQ3 may provide the best predictive measure of V[Combining Dot Above]O2peak from the 20-m shuttle run test in adolescents aged 11-13 years.