Plant cell cultures are widely used in the micro-propagation of clonal plants, especially virus-free plants, and in the production of useful metabolites such as paclitaxel. On the other hand, the use of plant cell cultures for the more basic characterization of plant functions is rather limited due to the difficulties associated with functional differentiation in cell cultures. In this review, I overview our experience with functionally differentiated cultured plant cells and their characteristics, especially with regard to photoautotrophism and secondary metabolism. I emphasize the high potential of functionally differentiated cell cultures, as well as some of the pitfalls, in the characterization of plant functions and biotechnological applications.