Enantioseparations by thin-layer chromatography

Methods Mol Biol. 2013:970:29-43. doi: 10.1007/978-1-62703-263-6_2.

Abstract

An up-to-date overview of thin-layer chromatography (TLC) techniques for chiral separations of various significant and/or recent examples of enantioresolutions is reported. Furthermore, examples for chiral separations obtained on achiral commercially available C18 TLC plates are described in detail. These include the enantioseparation of methylthiohydantoin-phenylalanine and methylthiohydantoin-tyrosine using hydroxyethyl-β-cyclodextrin as mobile phase additive and the separation of the enantiomers of warfarin and p-chlorowarfarin using bovine serum albumin as mobile phase additive.

MeSH terms

  • Animals
  • Cattle
  • Cellulose / analysis
  • Cellulose / chemistry
  • Chromatography, Thin Layer / methods*
  • Molecular Imprinting / methods
  • Phenylalanine / analysis
  • Phenylalanine / chemistry
  • Polymers / analysis
  • Polymers / chemistry
  • Serum Albumin, Bovine / analysis
  • Serum Albumin, Bovine / metabolism
  • Thiohydantoins / analysis
  • Thiohydantoins / chemistry
  • Tyrosine / analysis
  • Tyrosine / chemistry
  • Warfarin / analysis
  • Warfarin / chemistry
  • beta-Cyclodextrins / analysis
  • beta-Cyclodextrins / metabolism

Substances

  • Polymers
  • Thiohydantoins
  • beta-Cyclodextrins
  • Serum Albumin, Bovine
  • Tyrosine
  • Phenylalanine
  • Warfarin
  • methylthiohydantoin
  • Cellulose