Abstract
The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
Publication types
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Multicenter Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens / chemistry
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Allergens / immunology*
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Antigens, Plant / immunology*
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Carrier Proteins / immunology*
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Cross Reactions / immunology
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Electrophoresis, Polyacrylamide Gel
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Epitopes / chemistry
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Food
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Food Hypersensitivity / immunology
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Geography
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Humans
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Immunoassay / methods
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Lipids / chemistry
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Models, Statistical
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Plant Proteins / immunology*
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Pollen
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Protein Array Analysis / methods*
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Recombinant Proteins / chemistry
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Spain
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Allergens
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Antigens, Plant
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Carrier Proteins
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Epitopes
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Lipids
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Plant Proteins
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Recombinant Proteins
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lipid transfer proteins, plant
Grants and funding
Ministerio de Ciencia e Innovación (projects BIO2009-07050 and SAF2008-04053) and FIS-Thematic Networks, and Co-operative Research Centers: RIRAAF (RD07/0064). C Gómez Casado and L Tordesillas have been supported by training grants from the Spanish Government (FPI and FPU programmes, MEC, respectively). J Aguirre has been supported by the Ministerio de Ciencia e Innovación (project FIS2011-27569) and Comunidad de Madrid, R&D Program of Activities, grant MODELICO-CM S2009ESP-1691. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.