Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)

J Vet Sci. 2012 Dec;13(4):429-32. doi: 10.4142/jvs.2012.13.4.429.

Abstract

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / genetics
  • Blotting, Western / veterinary
  • DNA Primers / genetics
  • Deer / immunology*
  • Deer / metabolism*
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Endopeptidase K
  • Mice
  • Mice, Knockout
  • Polymerase Chain Reaction / veterinary
  • Prions / genetics
  • Prions / immunology*
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology*
  • Wasting Disease, Chronic / diagnosis*
  • Wasting Disease, Chronic / immunology*

Substances

  • Antibodies, Monoclonal
  • DNA Primers
  • Prions
  • Protein Isoforms
  • Endopeptidase K