Linezolid dependence in Staphylococcus epidermidis bloodstream isolates

Emerg Infect Dis. 2013 Jan;19(1):129-32. doi: 10.3201/eid1901.111527.

Abstract

We document linezolid dependence among 5 highly linezolid-resistant (LRSE) Staphylococcus epidermidis bloodstream isolates that grew substantially faster at 32 µg/mL linezolid presence. These isolates carried the mutations T2504A and C2534T in multiple 23S rRNA copies and 2 mutations leading to relevant amino acid substitutions in L3 protein. Linezolid dependence could account for increasing LRSE emergence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetamides / metabolism
  • Acetamides / pharmacology*
  • Adult
  • Aged
  • Amino Acid Substitution
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / isolation & purification
  • Drug Resistance, Bacterial
  • Female
  • Greece / epidemiology
  • Humans
  • Linezolid
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Oxazolidinones / metabolism
  • Oxazolidinones / pharmacology*
  • Point Mutation
  • RNA, Ribosomal, 23S / genetics*
  • RNA, Ribosomal, 23S / isolation & purification
  • Ribosomal Protein L3
  • Ribosomal Proteins / genetics
  • Serotyping
  • Staphylococcal Infections / blood
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology
  • Staphylococcus epidermidis / genetics
  • Staphylococcus epidermidis / growth & development*
  • Staphylococcus epidermidis / isolation & purification
  • Staphylococcus epidermidis / metabolism

Substances

  • Acetamides
  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Oxazolidinones
  • RNA, Ribosomal, 23S
  • Ribosomal Protein L3
  • Ribosomal Proteins
  • Linezolid