Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Fish Shellfish Immunol. 2013 Feb;34(2):724-8. doi: 10.1016/j.fsi.2012.11.041. Epub 2012 Dec 17.

Abstract

Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Surface Display Techniques / methods
  • DNA Primers / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli
  • Flounder
  • Immunoglobulin Variable Region / immunology*
  • Molecular Sequence Data
  • Novirhabdovirus / immunology*
  • Receptors, Antigen / immunology*
  • Sequence Analysis, DNA
  • Viral Proteins / immunology*

Substances

  • DNA Primers
  • Immunoglobulin Variable Region
  • Receptors, Antigen
  • Viral Proteins