Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

Laura van Dommelen; Petra F G Wolffs; Frank H van Tiel; Nicole Dukers; Selma B Herngreen; Cathrien A Bruggeman; Christian J P A Hoebe

doi:10.1128/JCM.02631-12

Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR

J Clin Microbiol. 2013 Mar;51(3):990-2. doi: 10.1128/JCM.02631-12. Epub 2012 Dec 19.

Authors

Laura van Dommelen¹, Petra F G Wolffs, Frank H van Tiel, Nicole Dukers, Selma B Herngreen, Cathrien A Bruggeman, Christian J P A Hoebe

Affiliation

¹ Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands.

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Chlamydia Infections / diagnosis
Chlamydia trachomatis / genetics
Chlamydia trachomatis / isolation & purification*
Culture Media / chemistry
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification*
Female
Humans
Real-Time Polymerase Chain Reaction / methods*
Specimen Handling / methods*
Temperature
Time Factors
Urine / microbiology
Vagina / virology

Substances

Culture Media
DNA, Bacterial