Abstract
A new withanolide, named withawrightolide (1), and four known withanolides (2-5) were isolated from the aerial parts of Datura wrightii. The structure of compound 1 was elucidated through 2D NMR and other spectroscopic techniques. In addition, the structure of withametelin L (2) was confirmed by X-ray crystallographic analysis. Using MTS viability assays, withanolides 1-5 showed antiproliferative activities against human glioblastoma (U251 and U87), head and neck squamous cell carcinoma (MDA-1986), and normal fetal lung fibroblast (MRC-5) cells with IC50 values in the range between 0.56 and 5.6 μM.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Carcinoma, Squamous Cell / drug therapy
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Crystallography, X-Ray
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Datura / chemistry*
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Drug Screening Assays, Antitumor
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Fibroblasts / drug effects
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Glioblastoma / drug therapy
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Humans
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Inhibitory Concentration 50
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Kansas
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Lung / cytology
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Lung / drug effects
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Withanolides / chemistry
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Withanolides / isolation & purification*
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Withanolides / pharmacology*
Substances
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Antineoplastic Agents, Phytogenic
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Withanolides