Engineering cell surfaces for orthogonal selectability

Yingjie Peng; Dae Hee Kim; Teresa M Jones; Diana I Ruiz; Richard A Lerner

doi:10.1002/anie.201201844

Engineering cell surfaces for orthogonal selectability

Angew Chem Int Ed Engl. 2013 Jan 2;52(1):336-40. doi: 10.1002/anie.201201844. Epub 2012 Dec 12.

Authors

Yingjie Peng¹, Dae Hee Kim, Teresa M Jones, Diana I Ruiz, Richard A Lerner

Affiliation

¹ Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.

PMID: 23239476
DOI: 10.1002/anie.201201844

Abstract

Vectors have been constructed that express the chitin-binding domain (ChBD) on eukaryotic cell surfaces. The ChBD is linked to enhanced green fluorescent protein (EGFP) through a protein that spans the plasma membrane. This binding functionality does not have a counterpart in eukaryotes, thereby endowing the modified cell surface with a property that is orthogonal to animal cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Engineering
Chitinases / chemistry*
Chitinases / metabolism
Gene Expression
HEK293 Cells
Humans
Protein Binding
Protein Engineering / methods*
Transfection

Substances

Chitinases