Resolution of a paradox by native mass spectrometry: facile occupation of all four metal binding sites in the dimeric zinc sensor SmtB

Chem Commun (Camb). 2013 Jan 28;49(8):813-5. doi: 10.1039/c2cc38387j.

Abstract

The predominant species of the cyanobacterial metalloregulatory protein SmtB as observed by ESI-MS is a dimer with all four zinc binding sites occupied.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Models, Molecular
  • Protein Multimerization
  • Spectrometry, Mass, Electrospray Ionization
  • Synechococcus / chemistry*
  • Synechococcus / metabolism
  • Zinc / metabolism*

Substances

  • Bacterial Proteins
  • Zinc