Abstract
Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basic Helix-Loop-Helix Transcription Factors / metabolism
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Cryptochromes / metabolism
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Gene Expression Regulation, Fungal / genetics
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Gene Expression Regulation, Fungal / radiation effects
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Light
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Optogenetics / methods*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / radiation effects
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Transcription, Genetic / genetics*
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Transcription, Genetic / radiation effects*
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Zebrafish / genetics*
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Zebrafish / metabolism
Substances
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Basic Helix-Loop-Helix Transcription Factors
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Cryptochromes