Simple paper architecture modifications lead to enhanced sensitivity in nanoparticle based lateral flow immunoassays

Lab Chip. 2013 Feb 7;13(3):386-90. doi: 10.1039/c2lc41144j.

Abstract

Lateral flow immunoassays (LFIA) are ideal biosensors to detect proteins, but their lack of sensitivity hinders their extensive use. We report a strategy that yields up to an 8-fold improvement in the sensitivity of a gold nanoparticles-based LFIA by changing the sizes of the pads. Theoretical flow simulations of the developed LFIA architectures are in accordance with the experimental results.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods*
  • Computer Simulation
  • Equipment Design
  • Gold / chemistry*
  • Humans
  • Immunoassay / methods
  • Immunoglobulin G / analysis
  • Metal Nanoparticles / chemistry*
  • Microscopy, Electron, Scanning
  • Models, Theoretical

Substances

  • Immunoglobulin G
  • Gold