Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy

J Biophotonics. 2014 May;7(5):287-94. doi: 10.1002/jbio.201200075. Epub 2012 Nov 28.

Abstract

In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D β -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58 , ST98 carrying blaOXA-24/40 and ST92 carrying blaOXA-23 . Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates.

Keywords: Acinetobacter baumannii; carbapenems; chemometrics; infrared spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / cytology
  • Acinetobacter baumannii / drug effects*
  • Acinetobacter baumannii / isolation & purification*
  • Anti-Bacterial Agents / pharmacology*
  • Carbapenems / pharmacology*
  • Clone Cells / drug effects
  • Discriminant Analysis
  • Drug Resistance, Bacterial*
  • Humans
  • Least-Squares Analysis
  • Spectroscopy, Fourier Transform Infrared*

Substances

  • Anti-Bacterial Agents
  • Carbapenems