Lateral shearing digital holographic imaging of small biological specimens

Opt Express. 2012 Oct 8;20(21):23617-22. doi: 10.1364/OE.20.023617.

Abstract

A lateral shearing interferometer is used for direct holographic imaging of microorganisms. This is achieved by increasing the shear to be larger than the object size and results in a very simple and inexpensive common-path imaging device that can be easily coupled to the output of an inverted microscope. The shear is created by reflections from the front and back surface of a glass plate. Stability measurements show a standard deviation of the phase measurements of less than 1nm over 8 min. without any vibration compensation. The setup is applied to imaging both microorganisms in a microfluidic channel and red blood cells and reconstructions are presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Flow Velocity / physiology
  • Cell Tracking / instrumentation*
  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Erythrocytes / cytology*
  • Erythrocytes / physiology*
  • Holography / instrumentation*
  • Humans
  • Interferometry / instrumentation*
  • Microfluidic Analytical Techniques / instrumentation*
  • Microscopy / instrumentation*
  • Shear Strength / physiology