Abstract
A diselenide containing fluorescent probe based on a fluorescein scaffold for thiols was developed. The fluorescent probe exhibited rapid response, high selectivity and reversibility. Confocal fluorescence microscopy was used to visualize the redox changes mediated by thiols and reactive oxygen species in living HeLa cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chemistry Techniques, Analytical / methods*
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Fluorescent Dyes / chemistry*
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HeLa Cells
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Humans
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Hydrogen Peroxide / chemistry*
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Microscopy, Confocal
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Molecular Structure
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Oxidative Stress*
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Selenium / chemistry*
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Spectrometry, Fluorescence
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Sulfhydryl Compounds / analysis*
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Time Factors
Substances
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Fluorescent Dyes
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Sulfhydryl Compounds
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Hydrogen Peroxide
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Selenium