Abstract
We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Conjugation, Genetic
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Copper / metabolism
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Copper / toxicity*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial / drug effects
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Gene Order
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Genes, Bacterial*
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Molecular Sequence Data
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Plasmids*
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Pseudomonas syringae / drug effects*
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Pseudomonas syringae / genetics*
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Pseudomonas syringae / metabolism
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Sequence Analysis, DNA
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Transformation, Bacterial
Associated data
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GENBANK/JX645720
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GENBANK/JX645721