Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae

Appl Environ Microbiol. 2013 Feb;79(3):1028-33. doi: 10.1128/AEM.02644-12. Epub 2012 Nov 26.

Abstract

We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conjugation, Genetic
  • Copper / metabolism
  • Copper / toxicity*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial*
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial / drug effects
  • Gene Order
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Plasmids*
  • Pseudomonas syringae / drug effects*
  • Pseudomonas syringae / genetics*
  • Pseudomonas syringae / metabolism
  • Sequence Analysis, DNA
  • Transformation, Bacterial

Substances

  • DNA, Bacterial
  • Copper

Associated data

  • GENBANK/JX645720
  • GENBANK/JX645721