Abstract
SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10(-3) , 10(-2) and 10(-1) M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriophages / chemistry*
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Bacteriophages / genetics
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Bacteriophages / ultrastructure
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Biocompatible Materials / chemistry
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Biocompatible Materials / metabolism
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Bioengineering / methods*
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Calcium Chloride / chemistry*
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Capsid Proteins / chemistry*
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Capsid Proteins / genetics
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Cobalt / chemistry*
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Escherichia coli / genetics
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Macromolecular Substances / chemistry
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Macromolecular Substances / metabolism
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Microscopy, Atomic Force
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Microscopy, Electron, Scanning
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Static Electricity
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Surface Properties
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Virus Assembly / physiology
Substances
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Biocompatible Materials
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Capsid Proteins
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Macromolecular Substances
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Recombinant Proteins
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coat protein, Bacteriophage M13
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Cobalt
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cobaltous chloride
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Calcium Chloride