Abstract
Effusanin C, a constituent of Isodon japonicus, has been used in oriental countries as a traditional folk medicine to treat inflammatory diseases, but its mechanism of action remains unknown. Here, we investigate the inhibitory activity of effusanin C in inflammatory monocytes. Effusanin C markedly inhibited the production of inflammatory mediators including nitric oxide, IL-1β, and TNF-α in macrophages and dendritic cells. Furthermore, molecular studies showed that effusanin C inhibited phosphorylation of p38, JNK, and ERK, degradation of IκBβ, and nuclear translocation of NF-κB p50/p65 in these cells. Taken together, these data show that effusanin C inhibits inflammatory responses by blocking NF-κB and MAPK signalings in monocytes.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Cell Line
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Dendritic Cells / drug effects
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Dendritic Cells / metabolism
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Diterpenes / pharmacology*
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Female
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Interleukin-1beta / genetics
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Isodon
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Mice, Inbred C57BL
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Mitogen-Activated Protein Kinases / antagonists & inhibitors*
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Mitogen-Activated Protein Kinases / metabolism
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Monocytes / drug effects
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Monocytes / metabolism
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / metabolism*
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Plant Components, Aerial
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Protein Kinase Inhibitors / pharmacology*
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Tumor Necrosis Factor-alpha / genetics
Substances
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Anti-Inflammatory Agents
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Diterpenes
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Interleukin-1beta
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NF-kappa B
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Protein Kinase Inhibitors
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Tumor Necrosis Factor-alpha
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effusanin C
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Mitogen-Activated Protein Kinases