Denitrification performance and microbial community structure of a combined WLA-OBCO system

PLoS One. 2012;7(11):e48339. doi: 10.1371/journal.pone.0048339. Epub 2012 Nov 9.

Abstract

The contamination of surface water by nitrogen due to fertilizer application and discharge of wastewater is an increasingly serious problem. A multifunctional device, which combines water-lifting and aeration (WLA) with oligotrophic biological contact oxidation (OBCO), was developed for pretreatment of raw water to reduce nitrogen. The performance of nitrogen removal and changes in microbial community structure were investigated. The results showed that the combined technique of WLA-OBCO was feasible, and that ammonium, nitrate, total nitrogen and total organic carbon were effectively removed. Meanwhile, nitrite was mostly undetectable. The PCR-DGGE and clone sequencing results revealed that α-proteobacterium was the largest bacterial group, and Pseudomonas strains Y3 and J8 were the dominant bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Biodegradation, Environmental
  • Biofilms / growth & development
  • Denitrification*
  • Nitrogen / chemistry*
  • Water Microbiology*
  • Water Pollutants, Chemical / chemistry*
  • Water Pollution, Chemical*
  • Water Purification / methods*

Substances

  • Water Pollutants, Chemical
  • Nitrogen

Grants and funding

This work was financially supported by the National Natural Science Foundation of China (NSFC)(Grant No. 50830303), the National Science & Technology Pillar Program (Grant No. 2012BAC04B02), and also by Shaanxi Provincial Science & Technology Innovative Project (Grant No. 2011KTCG03-07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.