Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter

L Carter; L A Lindsey; C J Grim; V Sathyamoorthy; K G Jarvis; G Gopinath; C Lee; J A Sadowski; L Trach; M Pava-Ripoll; B A McCardell; B D Tall; L Hu

doi:10.1128/AEM.02898-12

Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter

Appl Environ Microbiol. 2013 Jan;79(2):734-7. doi: 10.1128/AEM.02898-12. Epub 2012 Nov 9.

Authors

L Carter¹, L A Lindsey, C J Grim, V Sathyamoorthy, K G Jarvis, G Gopinath, C Lee, J A Sadowski, L Trach, M Pava-Ripoll, B A McCardell, B D Tall, L Hu

Affiliation

¹ CFSAN, FDA, Laurel, Maryland, USA.

Abstract

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Cronobacter / classification*
Cronobacter / enzymology
Cronobacter / genetics*
Cronobacter / isolation & purification
Escherichia coli Proteins / genetics*
Food Microbiology
Foodborne Diseases / diagnosis
Foodborne Diseases / microbiology
Multiplex Polymerase Chain Reaction / methods*
Phosphorus-Oxygen Lyases / genetics*
Sensitivity and Specificity

Substances

Escherichia coli Proteins
Phosphorus-Oxygen Lyases
diguanylate cyclase