Triple-helical recognition of RNA using 2-aminopyridine-modified PNA at physiologically relevant conditions

Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12593-6. doi: 10.1002/anie.201207925. Epub 2012 Nov 4.

Abstract

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases form stable and sequence-selective triple helices with double-stranded RNA at physiologically relevant conditions. The M-modified PNA showed unique RNA selectivity by having two orders of magnitude higher affinity for the double-stranded RNAs than for the same DNA sequences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aminopyridines / chemistry*
  • Base Pairing
  • MicroRNAs / chemistry
  • Nucleic Acid Conformation
  • Peptide Nucleic Acids / chemistry*
  • RNA, Double-Stranded / chemistry*

Substances

  • Aminopyridines
  • MicroRNAs
  • Peptide Nucleic Acids
  • RNA, Double-Stranded
  • alpha-aminopyridine