Optimal detection of serum antipaternal antileukocytic antibodies after injection of allogenic lymphocytes in women with habitual abortions

L V Krechetova; M A Nikolaeva; L V Van'ko; M M Ziganshina; E L Golubeva; E O Stepanova; G T Sukhikh

doi:10.1007/s10517-012-1811-8

Optimal detection of serum antipaternal antileukocytic antibodies after injection of allogenic lymphocytes in women with habitual abortions

Bull Exp Biol Med. 2012 Sep;153(5):726-9. doi: 10.1007/s10517-012-1811-8.

Authors

L V Krechetova¹, M A Nikolaeva, L V Van'ko, M M Ziganshina, E L Golubeva, E O Stepanova, G T Sukhikh

Affiliation

¹ VI Kulakov Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow, Russia. k_l_v@mail.ru

PMID: 23113270
DOI: 10.1007/s10517-012-1811-8

Abstract

The level of antipaternal antileukocytic antibodies detected by flow cytometry is a parameter reflecting the efficiency of alloimmunization of women with reproductive disorders during preparation to pregnancy. The results of evaluation of antipaternal antileukocytic antibodies by two modifications of the method are presented. The optimal method for detection of antipaternal antileukocytic antibodies after immunocytotherapy is selected.

MeSH terms

Abortion, Habitual / blood*
Abortion, Habitual / prevention & control*
Antilymphocyte Serum / isolation & purification*
Female
Flow Cytometry / methods
Humans
Immunization / methods*
Lymphocyte Transfusion / methods*
Male
Monocytes / immunology
Pregnancy
Transplantation, Homologous / methods*

Substances

Antilymphocyte Serum