Eleven diverse nuclear-encoded phylogenetic markers for the subfamily Panicoideae (Poaceae)

Am J Bot. 2012 Nov;99(11):e443-6. doi: 10.3732/ajb.1200186. Epub 2012 Oct 29.

Abstract

Premise of the study: Polyploidy is common in the grasses and low-copy nuclear loci are needed to further our understanding of phylogenetic relationships.

Methods and results: Genetic and genomic resources were combined to identify loci known to influence plant and inflorescence architecture. Degenerate primers were designed and tested to amplify regions of 11 nuclear-encoded loci across the panicoid grasses.

Conclusions: The primers designed in this study amplify regions of a diverse set of genes within the panicoid grasses. Properly employed, these markers will allow the identification of allopolyploid taxa and their diploid progenitors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Nucleus / genetics*
  • DNA Primers / genetics
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • Genes, Plant / genetics*
  • Genetic Variation
  • Molecular Sequence Data
  • Phylogeny*
  • Poaceae / classification
  • Poaceae / genetics*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Plant

Associated data

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