Abstract
Platelets are currently acknowledged as cells of innate immunity and inflammation and play a complex role in sepsis. We examined whether different types of LPS have different effects on the release of soluble signaling/effective molecules from platelets. We used platelet-rich plasma from healthy volunteers and LPS from two strains of gram-negative bacteria with disparate LPS structures. We combined LPS-stimulated platelet supernatants with reporter cells and measured the PBMC cytokine secretion profiles. Upon stimulation of platelets with both Escherichia coli O111 and Salmonella minnesota LPS, the platelet LPS::TLR4 interaction activated pathways to trigger the production of a large number of molecules. The different platelet supernatants caused differential PBMC secretion of IL-6, TNFα, and IL-8. Our data demonstrate that platelets have the capacity to sense external signals differentially through a single type of pathogen recognition receptor and adjust the innate immune response appropriately for pathogens exhibiting different types of 'danger' signals.
Copyright © 2012 Elsevier Inc. All rights reserved.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Blood Platelets / drug effects
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Blood Platelets / immunology*
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Cytokines / blood*
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Escherichia coli / immunology
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Humans
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Immunity, Innate / drug effects
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In Vitro Techniques
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Interleukin-6 / blood
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Interleukin-8 / blood
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Leukocytes, Mononuclear / immunology
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Lipopolysaccharide Receptors / blood
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Lipopolysaccharides / immunology*
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Lipopolysaccharides / isolation & purification
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Lipopolysaccharides / pharmacology
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P-Selectin / blood
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Platelet Activation / drug effects
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Platelet Activation / immunology
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Protein Isoforms / immunology
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Protein Isoforms / isolation & purification
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Protein Isoforms / pharmacology
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Salmonella / immunology
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Signal Transduction / immunology
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Species Specificity
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Tetraspanin 30 / blood
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Toll-Like Receptor 4 / blood*
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Tumor Necrosis Factor-alpha / blood
Substances
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CD63 protein, human
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CXCL8 protein, human
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Cytokines
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IL6 protein, human
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Interleukin-6
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Interleukin-8
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Lipopolysaccharide Receptors
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Lipopolysaccharides
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P-Selectin
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Protein Isoforms
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SELP protein, human
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TLR4 protein, human
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Tetraspanin 30
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Toll-Like Receptor 4
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Tumor Necrosis Factor-alpha