Abstract
Order in disorder: The characterization of intrinsically disordered proteins by NMR spectroscopy is a necessity on the one hand and a continuous challenge on the other. We propose two experiments that provide diagnostic parameters to monitor the degree of unfolding of a polypeptide. The test was performed on the yeast Cox17 protein, known to gain its function through maturation from an intrinsically disordered state (see figure).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cation Transport Proteins / chemistry*
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Copper Transport Proteins
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Disulfides / chemistry
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Models, Molecular
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Molecular Chaperones / chemistry*
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Nuclear Magnetic Resonance, Biomolecular*
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Oxidation-Reduction
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Protein Conformation
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Protein Unfolding*
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Saccharomyces cerevisiae / chemistry*
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Saccharomyces cerevisiae Proteins / chemistry*
Substances
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COX17 protein, S cerevisiae
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Cation Transport Proteins
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Copper Transport Proteins
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Disulfides
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Molecular Chaperones
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Saccharomyces cerevisiae Proteins