Genotyping of rotaviruses detected in children admitted to hospital from Faisalabad Region, Pakistan

J Med Virol. 2012 Dec;84(12):2003-7. doi: 10.1002/jmv.23402.

Abstract

Rotavirus infection is very common in developing countries and occurs at least once in children under the age of 5 years. The rate of detection of rotavirus infection in various age groups (0-5 years) in patients with gastroenteritis admitted to hospital from the Faisalabad region, Pakistan is reported. Out of 300 fecal samples, 189 (57.3%) were positive for rotavirus by immunoassay. Patients aged 7-12 months (35.4%) were infected most commonly followed by the age group 0-6 months (28%). Different genotypes of rotavirus were identified by hemi-nested RT-PCR. The most common genotype was G1P[8] (25.3%), followed by G1P[6] (21.1%). Other genotypes were G1P[9], G2P[6], G9P[10]), G3P[8] (1.5%), and G9P[11] (1%). There were two (1%) cases of mixed G genotype, one patient with two genotypes G1, G10 and another patient with 3 genotypes G1, G10, and G12. There were 6 (3.1%) cases of mixed P genotypes, 3 P[4], P[11] and 3 P[8], P[11]. These results provide an outline of rotavirus infection in this area for the first time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child, Preschool
  • Feces / virology
  • Female
  • Genotype*
  • Genotyping Techniques
  • Hospitalization
  • Hospitals
  • Humans
  • Infant
  • Male
  • Pakistan / epidemiology
  • Prevalence
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / virology

Substances

  • RNA, Viral