Enhancement of fatty acid-based polyurethanes cytocompatibility by non-covalent anchoring of chondroitin sulfate

Abstract

For tissue engineering purpose biopolymer chondroitin sulfate (CS), one of the major components of cartilage and bone extracellular matrix, was immobilized onto the surface of amino-functionalized polyurethane (PU) films derived from naturally occurring oleic and 10-undecenoic acids. The amino-functionalized PUs were prepared by aminolysis with 1,6-hexamethylenediamine of synthesized PUs containing methyl ester groups. FTIR-ATR, XPS, SEM, and water contact angle measurements were used to confirm the surface changes at each step of treatment, both in morphologies and chemical composition. Cytotoxicity and cell morphology analysis using osteoblast cell line MG63 showed that PU-CS films are suitable materials for cell growth, spreading, and differentiation.