In vitro analysis of the efficacy of ultrasonic scalers and a toothbrush for removing bacteria from resorbable blast material titanium disks

J Periodontol. 2013 Aug;84(8):1191-8. doi: 10.1902/jop.2012.120369. Epub 2012 Oct 17.

Abstract

Background: A resorbable blast material (RBM) surface is reported to have a higher bone-to-implant contact percentage than machined surfaces, but modified surfaces with rougher textures have been shown to favor colonization by bacteria and development of peri-implantitis. Therefore, this in vitro study compares the effects of different instruments on surface roughness and removal of bacteria from RBM titanium implant disks.

Methods: RBM titanium disks were treated with various ultrasonic scaler tips and a toothbrush, and change in surface roughness was measured by confocal microscopy. The disks were incubated with bacteria, and instruments made of carbon or plastic, two metal ultrasonic scaler tips, or a toothbrush were used to remove the attached bacteria. The amount of remaining bacteria was evaluated using a crystal violet assay.

Results: The change in surface structure following different treatment modalities was analyzed by confocal microscopy. A statistically significant decrease in the arithmetic mean value of RBM surfaces (R(a)) was observed after treatment with an ultrasonic scaler with a metal tip. The use of a metal tip (rather than a carbon or plastic tip) and brushing with dentifrice was more efficient in removing bacteria from the contaminated titanium surface according to the crystal violet assay.

Conclusion: Within the limits of this study, the use of a metal tip may be effective in removing bacteria from contaminated surfaces.

Publication types

  • Comparative Study

MeSH terms

  • Alloys / chemistry
  • Bacterial Adhesion*
  • Bacterial Load
  • Carbon / chemistry
  • Coated Materials, Biocompatible / chemistry*
  • Coloring Agents
  • Dental Materials / chemistry*
  • Dental Scaling / instrumentation*
  • Dentifrices / chemistry
  • Equipment Design
  • Gentian Violet
  • Humans
  • Materials Testing
  • Microscopy, Confocal
  • Plastics / chemistry
  • Porphyromonas gingivalis / isolation & purification
  • Porphyromonas gingivalis / physiology
  • Surface Properties
  • Titanium / chemistry*
  • Toothbrushing / instrumentation*
  • Ultrasonics / instrumentation*

Substances

  • Alloys
  • Coated Materials, Biocompatible
  • Coloring Agents
  • Dental Materials
  • Dentifrices
  • Plastics
  • Carbon
  • Titanium
  • Gentian Violet