Plant growth and cultivation

Methods Mol Biol. 2013:953:23-45. doi: 10.1007/978-1-62703-152-3_2.

Abstract

There is a variety of methods used for growing plants indoor for laboratory research. In most cases plant research requires germination and growth of plants. Often, people have adapted plant cultivation protocols to the conditions and materials at hand in their own laboratory and growth facilities. Here I will provide a guide for growing some of the most frequently used plant species for research, i.e., Arabidopsis thaliana, barley (Hordeum vulgare) and rice (Oryza sativa). However, the methods presented can be used for other plant species as well, especially if they are related to the above-mentioned species. The presented methods include growing plants in soil, hydroponics, and in vitro on plates. This guide is intended as a starting point for those who are just beginning to work on any of the above-mentioned plant species. Methods presented are to be taken as suggestive and modification can be made according to the conditions existing in the host laboratory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorine
  • Hydroponics
  • Plant Development*
  • Seeds / growth & development
  • Soil
  • Sterilization
  • Tissue Culture Techniques / methods*

Substances

  • Soil
  • Chlorine