Clonal differences in antioxidant activity and bioactive constituents of hardy kiwifruit (Actinidia arguta) and its year-to-year variability

J Sci Food Agric. 2013 Apr;93(6):1412-9. doi: 10.1002/jsfa.5909. Epub 2012 Oct 15.

Abstract

Background: Hardy kiwifruit (Actinidia arguta) is a new species, commercially grown in recent years. Total phenolics (TPC), vitamin C (TAA) content, antioxidant activity (AA) and their year-to-year variability in seven hardy kiwifruit clones were evaluated. TPC was determined using the Folin-Ciocalteu reagent assay. TAA was estimated by determination of l-ascorbic acid and l-dehydroascorbic acid levels using high-performance liquid chromatography. AA was measured using diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and OH radicals.

Results: The highest content of vitamin C, in all seasons, was found in D11 (1447-2181 mg kg(-1) fresh weight) and phenolics for D11 and M1 clones (2583-3312 and 2228-3414 mg gallic acid equivalents kg(-1) fresh weight, respectively). TPC and TAA content showed significant differences between hardy kiwifruit clones and showed significant year-to-year variability. Each year, the level of AA was significantly higher for D11 (DPPH, ABTS). AA was strongly correlated with TPC and TAA content in Actinidia fruit.

Conclusion: Hardy kiwifruit are an important source of vitamin C and phenolics, which resulted in their good antioxidant potential. A significantly higher content of these compounds was found in fruit of hybrid origin, which suggests that A. purpurea × A. arguta clones may be useful genetic resources for further interspecific hybridization.

Keywords: antioxidant activity; hydroxyl radical; kiwiberry; phenol content; pro-health properties; vitamin C.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinidia / chemistry*
  • Actinidia / genetics
  • Antioxidants / analysis*
  • Antioxidants / pharmacology
  • Ascorbic Acid / analysis*
  • Benzothiazoles / metabolism
  • Biphenyl Compounds / metabolism
  • Chimera
  • Diet
  • Fruit / chemistry*
  • Humans
  • Hydroxyl Radical / metabolism
  • Phenols / analysis*
  • Picrates / metabolism
  • Seasons*
  • Species Specificity
  • Sulfonic Acids / metabolism

Substances

  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Phenols
  • Picrates
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Hydroxyl Radical
  • 1,1-diphenyl-2-picrylhydrazyl
  • Ascorbic Acid