Abstract
Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides containing fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biologically active small ligands, tetracycline, dopamine and streptomycin.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Aptamers, Nucleotide / chemistry*
-
Benzopyrans / chemistry
-
Biosensing Techniques* / methods
-
Dopamine / analysis
-
Fluorescent Dyes / chemistry*
-
Molecular Sequence Data
-
Naphthols / chemistry
-
Peptides / chemistry*
-
Rhodamines / chemistry
-
Spectrometry, Fluorescence / methods
-
Streptomycin / analysis
-
Tetracycline / analysis
Substances
-
Aptamers, Nucleotide
-
Benzopyrans
-
Fluorescent Dyes
-
Naphthols
-
Peptides
-
Rhodamines
-
seminaphthorhodaminefluoride
-
Tetracycline
-
Dopamine
-
Streptomycin