Abstract
This study was undertaken to investigate the role of peroxynitrite (ONOO(-)) modified thymine-5'-monophosphate (TMP) in the generation of anti-DNA autoantibodies in patients with systemic lupus erythematosus (SLE). TMP was exposed to ONOO(-) in vitro and challenged in vivo. TMP and ONOO(-)-modified-TMP were found to be nonimmunogenic in rabbits. TMP-linked-BSA and ONOO(-)-modified-TMP-BSA induced high titer antibodies. Induced antibodies against ONOO(-)-TMP-BSA show crossreactions with nucleic acids conformers. A high degree of specific binding by SLE autoantibodies with ONOO(-)-TMP-BSA was observed. Our novel results provide an important insight into the immunological basis of anti-DNA autoantibodies generation in SLE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Animals
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Antibodies, Antinuclear / blood
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Antibodies, Antinuclear / immunology*
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Antibody Specificity*
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Autoantibodies / blood
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Autoantibodies / immunology*
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Autoantigens / immunology
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Binding, Competitive
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Case-Control Studies
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DNA Damage / immunology
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Female
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Humans
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Immunoglobulin G / immunology
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Lupus Erythematosus, Systemic / blood
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Lupus Erythematosus, Systemic / immunology*
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Male
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Middle Aged
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Peroxynitrous Acid / immunology*
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Protein Binding
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Rabbits
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Serum Albumin, Bovine / immunology
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Thymidine Monophosphate / immunology*
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Young Adult
Substances
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Antibodies, Antinuclear
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Autoantibodies
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Autoantigens
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Immunoglobulin G
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Peroxynitrous Acid
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Serum Albumin, Bovine
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Thymidine Monophosphate