Human identification is one of the most challenging sciences. Recently, study of lip prints has become slightly fashionable in forensic field. The aim of the present work is to determine the pattern of lip prints and evaluate its uniqueness in a sample of Egyptian population. The study included 955 subjects (2-65 years old). Lip prints were taken by direct rolling methods against hard background and each lip print was divided into six areas to be examined by magnifying hand lens. Thereafter, they were scanned and examined by the Microsoft office picture manager program. The results revealed that the lip print for each individual was unique and the complete vertical pattern was the commonest type among males and females living in Dakahlia Governorate, Egypt. Sex could not be differentiated from the lip print. It can be concluded that lip print analysis is an ancillary tool for personal identification especially in criminal investigations.