Recognition of the let-7g miRNA precursor by human Lin28B

FEBS Lett. 2012 Nov 16;586(22):3986-90. doi: 10.1016/j.febslet.2012.09.034. Epub 2012 Oct 11.

Abstract

Mammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3' GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics (MD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites / genetics
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Hydrogen Bonding
  • Mass Spectrometry
  • MicroRNAs / chemistry*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Models, Molecular
  • Molecular Dynamics Simulation
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation*
  • Protein Binding
  • Protein Structure, Tertiary*
  • RNA Precursors / chemistry*
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • RNA-Binding Proteins
  • Sequence Homology, Amino Acid

Substances

  • DNA-Binding Proteins
  • LIN28B protein, human
  • MicroRNAs
  • RNA Precursors
  • RNA-Binding Proteins
  • mirnlet7 microRNA, human