Urinary mRNA expression of CCN2/CCN3 as a noninvasive marker for monitoring glomerular structure changes in nondiabetic chronic kidney disease

Biomarkers. 2012 Dec;17(8):714-20. doi: 10.3109/1354750X.2012.722229. Epub 2012 Oct 15.

Abstract

Background: We investigated whether urinary mRNA of connective tissue growth factor (CCN2) and nephroblastoma overexpressed gene (CCN3) can provide clinical insight into the management of patients with nondiabetic CKD.

Methods: Urinary mRNA expression of CCN2 and CCN3 were measured by Real-time PCR in 35 CKD patients and 12 controls.

Results: Urinary mRNA of CCN2 and CCN3 were distinctively greater in CKDs than healthy controls. Urinary CCN3/CCN2 mRNA ratio correlated to the degree of glomerular histological changes in those who received renal biopsy.

Conclusion: Urinary CCN3/CCN2 mRNA ratio may be a useful noninvasive biomarker for evaluating patients with nondiabetic CKD prior to renal biopsy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / urine*
  • Biopsy
  • Connective Tissue Growth Factor / genetics*
  • Female
  • Gene Expression
  • Glomerular Filtration Rate
  • Humans
  • Kidney / pathology
  • Kidney Glomerulus / pathology*
  • Male
  • Middle Aged
  • Nephroblastoma Overexpressed Protein / genetics*
  • Proteinuria / urine
  • RNA, Messenger / urine*
  • Renal Insufficiency, Chronic / diagnosis
  • Renal Insufficiency, Chronic / genetics
  • Renal Insufficiency, Chronic / urine*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Biomarkers
  • CCN2 protein, human
  • CCN3 protein, human
  • Nephroblastoma Overexpressed Protein
  • RNA, Messenger
  • Connective Tissue Growth Factor