Subcommunity FTIR-spectroscopy to determine physiological cell states

Curr Opin Biotechnol. 2013 Feb;24(1):88-94. doi: 10.1016/j.copbio.2012.09.008. Epub 2012 Oct 8.

Abstract

Estimation of growth potential in a complex community is a great challenge in biotechnical processes and environmental water quality control. Recently it has been shown that the macromolecular structure is a good indicator for the growth potential of phytoplankton cells. A functional understanding of natural phytoplankton communities requires a community analysis by means of single particles technologies. However, conventional biochemical methods are not sensitive enough to determine the macromolecular composition of a single cell or cell aggregates. This problem can be resolved by Fourier transform infrared (FTIR) spectroscopy, which delivers results similar to biochemical analysis with a much smaller sample size. The combined approach of flow cytometric analysis with subcommunity sorting and subsequent FTIR-analysis offers new perspectives for the understanding of community functioning and process optimization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Ecology / methods
  • Flow Cytometry
  • Phytoplankton / chemistry
  • Phytoplankton / cytology*
  • Phytoplankton / growth & development
  • Phytoplankton / metabolism*
  • Spectroscopy, Fourier Transform Infrared / methods*