Detection of Legionella bozemanae, a new cause of septic arthritis, by PCR followed by specific culture

J Clin Microbiol. 2012 Dec;50(12):4180-2. doi: 10.1128/JCM.01899-12. Epub 2012 Oct 10.

Abstract

Legionella bozemanae is a rare isolate in clinical specimens. We describe a case of joint infection due to L. bozemanae in an immunocompromised patient with dermatomyositis. Without the use of PCR screening or culture on specialized medium, the organism would not have been detected.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Arthritis, Infectious / diagnosis*
  • Arthritis, Infectious / microbiology*
  • Bacteriological Techniques / methods
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Dermatomyositis / complications
  • Female
  • Humans
  • Immunocompromised Host
  • Knee Joint / diagnostic imaging
  • Knee Joint / pathology
  • Legionella / classification*
  • Legionella / genetics
  • Legionella / growth & development
  • Legionella / isolation & purification*
  • Legionellosis / diagnosis*
  • Legionellosis / microbiology*
  • Multimodal Imaging
  • Polymerase Chain Reaction / methods*
  • Positron-Emission Tomography
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tomography, X-Ray Computed

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S