Mass-producible nano-featured polystyrene surfaces for regulating the differentiation of human adipose-derived stem cells

Macromol Biosci. 2012 Nov;12(11):1480-9. doi: 10.1002/mabi.201200225. Epub 2012 Oct 5.

Abstract

In this study, we report an efficient and cost-effective method of fabricating polystyrene (PS) nano-featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano-stamps. We investigate the behavior of adipose-derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo- and NPi-featured substrates do not alter the morphology of stem cells. However, both NPo- and NPi-featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo-featured substrate exhibit greater adipogenic differentiation, while the NPi-featured substrate induces higher osteogenic differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects*
  • Biocompatible Materials / chemical synthesis*
  • Biocompatible Materials / pharmacology
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Chondrogenesis / drug effects
  • Gene Expression
  • Humans
  • Integrins / genetics
  • Integrins / metabolism
  • Microscopy, Electron, Scanning
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Nickel / chemistry
  • Osteogenesis / drug effects
  • Polystyrenes / chemistry*
  • Stem Cells / cytology
  • Stem Cells / drug effects*
  • Surface Properties

Substances

  • Biocompatible Materials
  • Integrins
  • Polystyrenes
  • Nickel