Enabling Wittig reaction on site-specific protein modification

Chem Commun (Camb). 2012 Nov 21;48(90):11079-81. doi: 10.1039/c2cc35738k. Epub 2012 Oct 5.

Abstract

An efficient aqueous Wittig reaction was enabled on protein bioconjugation for the first time. By investigating the reaction on small molecules, peptides, and proteins, a site-specific reaction targeting "aldehyde tag" was presented. A variety of functional groups could be introduced into the protein of interest.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry
  • Interleukin-8 / chemistry
  • Interleukin-8 / metabolism
  • Peptides / chemistry
  • Peptides / metabolism
  • Periodic Acid / chemistry
  • Proteins / chemistry*
  • Proteins / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Aldehydes
  • Interleukin-8
  • Peptides
  • Proteins
  • Periodic Acid
  • metaperiodate