Effects of fullerene derivatives on bioluminescence and application for protease detection

Chem Commun (Camb). 2012 Nov 18;48(89):11011-3. doi: 10.1039/c2cc36099c.

Abstract

The effects of two C(60) derivatives: C(60)[C(COOH)(2)](3) and C(60)(NH(2))(x)(OH)(y), have been investigated on the bioluminescence of humanized Gaussia luciferase (hGluc) for the first time. Utilizing the dual properties of carboxyl C(60) with luminescence quenching and free radicals scavenging, a novel BRET system was constructed for protease detection with high sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fullerenes / chemistry*
  • Fullerenes / pharmacology
  • Limit of Detection
  • Luciferases / analysis*
  • Luciferases / drug effects
  • Luminescence
  • Molecular Structure
  • Peptide Hydrolases / analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Fullerenes
  • Luciferases
  • Peptide Hydrolases