A thorough knowledge of root canal anatomy is essential for successful root canal treatment, because the number of roots and root canals in any tooth can vary. Failing to detect an extra root or canal can cause root canal treatment to fail. It is essential that clinicians interpret radiographs correctly to detect any anatomic variations and treat them successfully. Anatomic variations occur frequently in the root canal system of maxillary premolars; however, the presence of three separate root canals with three independent roots is rare. This article presents an unusual case of three-rooted maxillary first and second premolars, each with three distinct root canals.