Entry and replication of recombinant hepatitis C viruses in cell culture

Gabrielle Vieyres; Thomas Pietschmann

doi:10.1016/j.ymeth.2012.09.005

Entry and replication of recombinant hepatitis C viruses in cell culture

Methods. 2013 Feb;59(2):233-48. doi: 10.1016/j.ymeth.2012.09.005. Epub 2012 Sep 23.

Authors

Gabrielle Vieyres¹, Thomas Pietschmann

Affiliation

¹ Institute of Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research; A Joint Venture Between The Medical School Hannover and The Helmholtz Centre for Infection Research, Feodor-Lynen-Straße 7-9, 30625 Hannover, Germany.

PMID: 23009812
DOI: 10.1016/j.ymeth.2012.09.005

Abstract

Hepatitis C virus (HCV) is a positive-strand enveloped RNA virus and belongs to the Flaviviridae family. The heavy health burden associated with the virus infection in humans and the intriguing peculiarities of the interaction between the HCV replication cycle and the hepatocyte host cell have stimulated a flourishing research field. The present review aims at recapitulating the different viral and cellular systems modelling HCV entry and replication, and in particular at gathering the tools available to dissect the HCV entry pathway.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cell Culture Techniques
Hepacivirus / physiology*
Humans
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Virus Internalization*
Virus Replication / genetics
Virus Replication / physiology*

Substances

Recombinant Proteins