[EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata]

Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(12):1731-3.
[Article in Chinese]

Abstract

Objective: To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata.

Method: Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized.

Result and conclusion: The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.

Publication types

  • English Abstract

MeSH terms

  • Cell Survival / drug effects
  • Cell Survival / genetics
  • Dose-Response Relationship, Drug
  • Ethyl Methanesulfonate / pharmacology*
  • Mutagenesis / drug effects
  • Mutation / drug effects*
  • Pinellia / cytology*
  • Pinellia / drug effects
  • Pinellia / genetics*
  • Pinellia / physiology
  • Suspensions
  • Temperature*
  • Time Factors

Substances

  • Suspensions
  • Ethyl Methanesulfonate