MAP kinases, MPK9 and MPK12, regulate chitosan-induced stomatal closure

Biosci Biotechnol Biochem. 2012;76(9):1785-7. doi: 10.1271/bbb.120228. Epub 2012 Sep 7.

Abstract

Chitosan (CHT)-induced stomatal closure was inhibited by an MAPKK inhibitor, PD98059, and was impaired in mpk9 mpk12 but not in mpk9 or mpk12. CHT induced the production of reactive oxygen species, cytosolic alkalization, and cytosolic Ca(2+) oscillation in mpk9 mpk12. These results suggest that MPK9 and MPK12 are involved in CHT-induced stomatal closure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / drug effects*
  • Arabidopsis / enzymology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Calcium / metabolism
  • Chitosan / pharmacology*
  • Cytosol / drug effects
  • Cytosol / metabolism
  • Fluoresceins
  • Fluorescent Dyes
  • Hydrogen-Ion Concentration
  • Mitogen-Activated Protein Kinase 12 / genetics
  • Mitogen-Activated Protein Kinase 12 / metabolism*
  • Mitogen-Activated Protein Kinase 9 / genetics
  • Mitogen-Activated Protein Kinase 9 / metabolism*
  • Mutation
  • Plant Stomata / drug effects*
  • Plant Stomata / enzymology
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • Signal Transduction / genetics

Substances

  • Arabidopsis Proteins
  • Fluoresceins
  • Fluorescent Dyes
  • Reactive Oxygen Species
  • 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester
  • Chitosan
  • Mitogen-Activated Protein Kinase 12
  • Mitogen-Activated Protein Kinase 9
  • Calcium