Biosynthesis of anti-HCV compounds using thermophilic microorganisms

Bioorg Med Chem Lett. 2012 Oct 1;22(19):6059-62. doi: 10.1016/j.bmcl.2012.08.045. Epub 2012 Aug 21.

Abstract

This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2'-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379mg/L of 6-chloropurine-2'-deoxyriboside. The obtained compounds can be used as antiviral agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / chemistry
  • Antiviral Agents / metabolism*
  • Antiviral Agents / pharmacology
  • Geobacillus stearothermophilus / chemistry
  • Geobacillus stearothermophilus / metabolism*
  • Hepacivirus / drug effects*
  • Purine Nucleosides / biosynthesis*
  • Purine Nucleosides / chemistry
  • Purine Nucleosides / pharmacology*
  • Temperature

Substances

  • 6-chloropurine-2'-deoxyriboside
  • Antiviral Agents
  • Purine Nucleosides
  • 6-chloropurine riboside