Detection of viable Mycobacterium ulcerans in clinical samples by a novel combined 16S rRNA reverse transcriptase/IS2404 real-time qPCR assay

PLoS Negl Trop Dis. 2012;6(8):e1756. doi: 10.1371/journal.pntd.0001756. Epub 2012 Aug 28.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteriological Techniques / methods
  • Buruli Ulcer / diagnosis*
  • Child
  • DNA Transposable Elements*
  • DNA, Bacterial / genetics*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods
  • Mycobacterium ulcerans / genetics
  • Mycobacterium ulcerans / isolation & purification*
  • RNA, Ribosomal, 16S / genetics*
  • Real-Time Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • DNA Transposable Elements
  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Grants and funding

The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement N° 241500. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.