Treatment of catheter-associated infections caused by Staphylococcus aureus is difficult without catheter removal, because of the biofilm formation and its high virulence. In this report, we presented our clinical and microbiological experience with systemic daptomycin (6 mg/kg/on alternate days IV) as well as antibiotic lock therapy (filling of the catheter lumen with 3.5 mg/ml daptomycin together with 0.045 mg/ml calcium) in the treatment of a catheter-associated bloodstream infection caused by methicillin-resistant S.aureus in a patient with hemodialysis catheter which could not be removed.